![]() Organic solvents such as acetone, methanol, and ethanol.Sugars such as sucrose, glucose, and fructose.Tissue culture media such as Eagle's MEM and Hank's salt solution.Buffers including HEPES, MES, MOPS, Tris, and phosphate.Reducing agents such a dithiothreitol and β-mercaptoethanol.Denaturing agents such as sodium thiocyanate, guanidine HCl, urea, and phenol.A standard Bradford protein assay kit is compatible with the following chemicals: The assay is quite robust and is compatible with many compounds commonly found in protein preparations. ![]() ![]() The other kit provides more flexibility with 5x concentrated reagent and lyophilized BSA or bovine-globulin standards, allowing the user to prepare reagent and standards at any concentration.Īll protein assays are subject to interference by certain substances under some conditions. One contains ready-to-use reagent and prediluted BSA or BGG standards for a simple and quick assay. Proteins commonly used as standards include bovine serum albumin (BSA) and bovine γ-globulin (BGG). The exact protein concentration of the sample is determined by interpolation from a standard curve made by measuring the absorbance of a dilution series of protein standards of known concentrations within the linear response range of the assay. The absorbance at 595 nm is then read either in a spectrophotometer or a microplate reader and is directly proportional to the amount of protein bound. When the Bradford reagent (acidified Coomassie Brilliant Blue G-250) binds to proteins, the dye undergoes a color change in the visible spectrum, with the absorbance maximum moving from 470 to 595 nm. The Bradford protein assay is a time-tested colorimetric assay.
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